De novo cell-free synthesis of picornavirus

US5674729A · kind A · utility

Assignee

• Research Foundation for the State University of New York York · US

Inventors

• Eckard Wimmer · Port Jefferson, US
• Akhteruzzaman Molla · Port Jefferson, US
• Aniko Paul · Setauket, US

Key dates

• Filing date: Jun 30, 1994
• Grant date: Oct 7, 1997
• Expiry date: Jun 30, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2770/32051
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process for the de novo synthesis of a picornavirus using a cell-free medium has been developed. The cell-free medium is prepared from a lysate of mammalian cells from which the nuclei and mitochondria has been removed, endogenous mRNA deactivated and supplemented with nucleoside triphosphates, tRNA, amino acids and precursors necessary for generating proteins. The genomic RNA of the picornavirus or rhinovirus is added to the cell-free medium and after incubation at about 30.degree. C. to 40.degree. C. of from about 4 to 24 hours infectious, mature virus particles are formed.