In vitro differentiation of CD34.sup.+ progenitor cells into T lymphocytes
US5677139A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 21, 1995 |
| Grant date | Oct 14, 1997 |
| Priority date | — |
| Expiry date | Apr 21, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2503/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention involves a method for the in vitro T cell production. A monolayer of non-human primate thymic stromal cells are cocultured in vitro with primate hematopoietic T cell progenitor cells. This results in the differentiation and growth of mature T cells. The T cells may be isolated at any sequential stage of differentiation and further expanded by coculture with a mitogenic agent. The T cells also may be genetically altered at any stage of the process. The effect of agents on the growth and differentiation of T cells may be measured by comparing a coculture containing the agent with a control coculture and comparing the differentiation or growth of the T cells progenitor cells in the test culture with the control culture. Kits and novel populations of T cells are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.