Biochemical assay plate and method for making the same
US5677197A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 14, 1994 |
| Grant date | Oct 14, 1997 |
| Priority date | — |
| Expiry date | Nov 14, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/809
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An assay system for detecting the binding of a mobile reactant to an immobilized reactant on an assay plate. The assay plate includes a substrate having an assay spot deposited thereon. The assay spot includes the immobilized reactant. In the present invention, a carrier dye is included in the assay spot, the amount of the carrier dye indicating the amount of the immobilized reactant that is present in the assay spot. In the preferred embodiment of the present invention, the assay spot is generated by depositing liquid on the assay plate at a location corresponding to the assay spot. The carrier dye is dissolved in the liquid and remains after the liquid evaporates. The amount of carrier dye in each spot may be measured spectroscopically and provides a means for identifying defective assay plates. The amount of carrier dye in each spot may also be recorded on the assay plate and used in conjunction with measurements of the amount of material bound to the assay spot to determine the concentration of a reactant in a solution being tested for the reactant.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.