Fibrin binding domain polypeptides and uses and methods of producing same

US5679320A · kind A · utility

Assignee

• Bio-Technology General Corporation · US

Inventors

• Tikva Vogel · Rehovot, IL
• Avigdor Levanon · Netanya, IL
• Moshe M. Werber · Nes Ziona, IL
• Rachel Guy · Rehovot, IL
• Amos Panet · Jerusalem, IL
• Jacob Hartman · Holon, IL
• Hadassa Shaked · Ramat Gan, IL

Key dates

• Filing date: Jun 14, 1994
• Grant date: Oct 21, 1997
• Expiry date: Jun 14, 2014

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K2123/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

This invention provides an imaging agent which comprises a polypeptide labeled with an imageable marker, such polypeptide having an amino acid sequence substantially present in the fibrin binding domain of naturally-occurring human fibronectin and being capable of binding to fibrin. The invention further provides a method wherein the imaging agent is used for imagifig a fibrin-containing substance, i.e., a thrombus or atherosclerotic plaque. Further provided are plasmids for expression of polypeptides having an amino acid sequence substantially present in the fibrin binding domain of naturally-occurring human fibronectin and being capable of binding to fibrin, hosts containing these plasmids, methods of producing the polypeptides, methods of treatment using the polypeptides, and methods of recovering, refolding and reoxidizing the polypeptides. The invention also provides for purified polypeptides substantially free of other substances of human origin which have an amino acid sequence substantially present in the fibrin binding domain of naturally-occurring human fibronectin and which are capable of binding to fibrin.