Threshold ligand-receptor assay
US5679526A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 1, 1994 |
| Grant date | Oct 21, 1997 |
| Priority date | — |
| Expiry date | Aug 1, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/825
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention is directed to a ligand-receptor assay for determining the presence of at least one target ligand, capable of competing with a ligand analogue conjugate for binding sites available on a ligand receptor, said ligand analogue conjugate comprising at least one ligand analogue coupled to a colloidal gold particle, in a fluid sample suspected of containing said target ligand comprising the steps of: PA1 a. contacting said fluid sample with said ligand analogue conjugate and said ligand receptor to form a homogeneous reaction mixture, the relative amounts of said ligand analogue conjugate and said ligand receptor being selected such that in the absence of said target ligand and subsequent to substantially equilibrium binding in said reaction mixture, substantially all of said ligand analogue conjugate is bound to said ligand receptor such that no unbound ligand analogue conjugate is detected as a result of the assay method; PA1 b. detecting unbound ligand analogue conjugates in said reaction mixture by visual means using at least one ligand receptor immobilized on a solid phase in at least one distinct locus; PA1 c. relating the detectable signal to the presence of said ta…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.