Particle-mediated transformation of gymnosperms
US5681730A · kind A · utility
19Cited by
4References
10Claims
0Family size
Assignee
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Key dates
| Filing date | Dec 21, 1993 |
| Grant date | Oct 28, 1997 |
| Priority date | — |
| Expiry date | Dec 21, 2013 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA01H4/005
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for genetically engineering gymnosperm tree species is based on accelerated particle transformation. Tissue of spruce is cultured into an embryogenic callus. Somatic embryos are produced by the callus and then subjected to an accelerated particle transformation process. The treated embryos are then induced to form embryogenic callus cultures and selected for the presence of gene products coded by the introduced genes. Somatic embryos are then induced from the callus and transgenic seedlings are produced.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.