Biotin-binding containment systems
US5681745A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | May 1, 1995 |
| Grant date | Oct 28, 1997 |
| Priority date | — |
| Expiry date | May 1, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/465
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to genetic containment systems which express a biotin-binding component that can be used for selectively destroying recombinant cells such as genetically engineered microorganisms. These systems may comprise a streptavidin or an avidin gene whose expression is controlled by a regulatable promoter. The regulatory agent such as a transcriptional effector is expressed from another gene which may also be expressed and its expression controlled by the containment system. Expression of the agent can be designed to respond to physiological changes in the environment. The invention also relates to containment systems and methods for the selective detection or tracking of recombinant cells and to eukaryotic and prokaryotic cells which contain these genetic containment systems.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.