Method for improving the performance of an immunoreagent in an immunoassay

US5681754A · kind A · utility

Assignee

• Abbott Laboratories · US

Inventors

• Mark R. Pope · Grayslake, US
• Peter Tarcha · Lake Villa, US
• David R. Mees · Chesterfield, US
• Mary K. Joseph · Libertyville, US
• Terry A. Pry · Libertyville, US
• C. Brent Putman · Round Lake Beach, US
• Daniel D. Subotich · Morton Grove, US

Key dates

• Filing date: Jun 7, 1996
• Grant date: Oct 28, 1997
• Expiry date: Jun 7, 2016

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/823
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for increasing the binding activity of specific binding members bound to a solid phase material, e.g., a particle, that has been sterically stabilized. This increase in binding activity is brought about by degrading a steric stabilizer on the surface of the solid phase material. The method involves both immobilizing a specific binding member on the surface of a solid phase material and degrading a steric stabilizer on the surface of that solid phase material. In the preferred embodiment, the method involves the immobilization of a specific binding member on the surface of the sterically stabilized solid phase material, with subsequent degradation of the steric stabilizer.