Method of identifying sequence in a nucleic acid target using interactive sequencing by hybridization
US5683881A · kind A · utility
30Cited by
1References
14Claims
0Family size
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Key dates
| Filing date | Oct 20, 1995 |
| Grant date | Nov 4, 1997 |
| Priority date | — |
| Expiry date | Oct 20, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A new approach is proposed for sequencing by hybridization (SBH), which uses interaction to dramatically reduce the number of oligonucleotides used for de novo sequencing of large DNA fragments, while preserving the parallelism which is the primary advantage of SBH. In particular, a series of rounds is performed, starting from an initial fixed oligonucleotide array, of hybridizing a target sample against an array, and then designing a new oligonucleotide array in response to the results of the rounds to date, until the sequence is determined.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.