Oligonucleotide decoys and methods relating thereto
US5683985A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 22, 1993 |
| Grant date | Nov 4, 1997 |
| Priority date | — |
| Expiry date | Sep 22, 2013 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61P35/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Improved DNA structures are disclosed which contain target sequences which bind to control proteins (such as the CREB protein). The structures of the present invention are stable to degradation, and are effective as decoys for control proteins, making it possible to modulate the transcriptional control normally exerted by such control proteins. In addition, there is provided a method to reversibly crosslink oligonucleotides to polypeptides which recognize the oligonucleotide sequence. This method involves synthesizing DNA structures as described above, wherein one or more phosphorothioate diester linkages are incorporated into the resulting oligonucleotide, allowing the phosphorothioate diester-containing oligonucleotide to bind to polypeptides which recognize the sequence of said oligonucleotide, then contacting the polypeptide-bound oligonucleotide with a transition metal reagent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.