Biosynthesis of zeaxanthin and glycosylated zeaxanthin in genetically engineered hosts

US5684238A · kind A · utility

Assignee

• Amoco Corporation · US

Inventors

• Rodney L. Ausich · Des Moines, US
• Friedhelm Brinkhaus · Des Moines, US
• Indrani Mukharji · Evanston, US
• John Proffitt · Oak Park, US
• James G. Yarger · Cedarburg, US
• Huei-Che Bill Yen · Naperville, US

Key dates

• Filing date: Jul 22, 1993
• Grant date: Nov 4, 1997
• Expiry date: Jul 22, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/825
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

DNA segments encoding the Erwinia herbicola enzymes geranylgeranyl pyrophosphate (GGPP) synthase, phytoene synthase, phytoene dehydrogenase-4H, lycopene cyclase, beta-carotene hydroxylase, and zeaxanthin glycosylase, DNA variants and analogs thereof encoding an enzyme exhibiting substantially the same biological activity, vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes, zeaxanthin and zeaxanthin diglucoside by recombinant DNA technology in transformed host organisms are disclosed.