Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
US5686279A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 10, 1994 |
| Grant date | Nov 11, 1997 |
| Priority date | — |
| Expiry date | Jun 10, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2740/13062
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The invention provides a novel retroviral packaging system, in which retroviral packaging constructs and packagable vector transcripts are produced from high expression plasmids by transfection in human cells. High titers of recombinant retrovirus are produced in infected cells. The methods of the invention include the use of the novel retroviral constructs to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation at high efficiencies. The invention is useful for the rapid production of high viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.