Establishment of new cell lines from Pseudaletia unipuncta with differential responses to baculovirus
US5686305A · kind A · utility
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Key dates
| Filing date | Feb 26, 1997 |
| Grant date | Nov 11, 1997 |
| Priority date | — |
| Expiry date | Feb 26, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2710/14122
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This disclosure presents the establishment of a new cell line from Pseudaletia unipuncta embryos. This cell line demonstrated the ability to produce high numbers of baculoviruses in cell culture. These virus particles are found internally in the cells in occlusion bodies. In the study of Pseudaletia unipuncta two baculoviruses were found to infect this species: P. unipuncta nuclear polyhedrosis virus (PuNPV), and P. unipuncta granulosis virus (PuGV). In addition, the cell line was also selected and cultured for its ability to grow in suspension while maintaining high levels of OB production.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.