Process for preparing human coagulation factor VIII protein complex
US5693499A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Jul 18, 1994 |
| Grant date | Dec 2, 1997 |
| Priority date | — |
| Expiry date | Jul 18, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2840/20
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A plasmid for expression of human coagulation factor VIII H-chain, a plasmid for expression of human coagulation factor VIII L-chain, an animal cell transformed with either said H-chain expression plasmid or said L-chain expression plasmid or with both thereof, and a process for preparing a human coagulation factor VIII protein complex which comprises forming a transformed animal cell by introducing both said H-chain expression plasmid and said L-chain expression plasmid into said animal cell, culturing said cell to produce the human coagulation factor VIII protein complex in the culture medium and collecting the same. The process of the present invention allows for the production of a safe factor VIII at a high expression level applicable for the production of Factor VIII on an industrial scale.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.