Methods for coating metal matrices for use in high gradient magnetic separation of biological materials and method for coating the same

US5693539A · kind A · utility

Inventors

• Stefan Miltenyi · Wittlich, DE
• Andreas Radbruch · Bonn, DE
• Walter Weichel · Cologne, DE
• Werner E.G. Müller · Cologne, DE
• Christoph Gottlinger · Cologne, DE
• Klaus Meyer · Dormagen, DE

Key dates

• Filing date: May 31, 1994
• Grant date: Dec 2, 1997
• Expiry date: May 31, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/807
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Improvements in the existing procedures and materials for conduct of high gradient magnetic separation (HGMS) are disclosed. Superior superparamagnetic particles, optionally coated with a polysaccharide or other, usually organic, materials can be prepared in uniform compositions with homogeneous magnetizations. The coating can conveniently be conjugated to a specific binding moiety complementary to a biological material whose purification or separation is desired. In addition, plastic coated matrices which form superior magnetic gradient-intensifying supports are disclosed, along with improved methods and apparatus to conduct HGMS.