Purification of DNA on Hydroxylated Silicas
US5693785A · kind A · utility
43Cited by
11References
19Claims
0Family size
Assignee
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Key dates
| Filing date | Nov 17, 1994 |
| Grant date | Dec 2, 1997 |
| Priority date | — |
| Expiry date | Nov 17, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Compositions and processes for isolation or purification of DNA are provided. The compositions are hydroxylated silica polymers produced by reacting silicon dioxide with an alkaline solution, followed by acidification. The hydroxylated silicas produced by this process can be used to bind DNA in aqueous solutions, without the need for binding reagents such as alcohols or chaotropes. The bound DNA may then be separated from the solution and eluted into water or a buffer by heating.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.