Patent · US Expired

Multilayer enzyme electrode membranes and methods of making same

US5696314A · kind A · utility

272Cited by
16References
55Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJul 12, 1996
Grant dateDec 9, 1997
Priority date
Expiry dateJul 12, 2016

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S435/817
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

An electrochemical sensor is designed for use in detecting a concentration of an analyte in a sample solution. The sensor to have a longer useful lifetime, improved stability and improved response time for single or multiple use applications. In one aspect, some of these goals are achieved by providing the multilayer enzyme electrode membrane having both nonporous and microporous layers. The microporous layer is formed from a polymer binder, a polymer powder, a surfactant and a mineral powder. In another aspect, certain of these goals are achieved by the use of a layer comprised of an enzyme disposed within a polymer matrix. In certain embodiments, the multilayer enzyme electrode membrane includes an outermost layer which is capable of dissolving in the sample solution such that the interface between the multilayer enzyme electrode membrane and the sample solution is continually renewed. The sensor provides an electrical current output proportional to the reagent chemical interaction of the analyte with an immobilized enzyme layer during exposure of the sensor in such solution.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.