Apparatus for staining of cells and tissues
US5698162A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 3, 1997 |
| Grant date | Dec 16, 1997 |
| Priority date | — |
| Expiry date | Feb 3, 2017 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2400/0406
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A slide for reacting a biological sample with reagents, and a method for using the same. The slide comprises a liquid-impervious top surface comprising glass, first portions of the surface having thereon a hydrophobic mask that repels an aqueous liquid, and second portions of the surface being completely free of any coverage by the mask so as to be wettable with an aqueous liquid, at least one of the other wettable second portions being totally isolated from the other wettable second portions by some of the mask, so that aqueous liquid will not flow from the one wettable portion to the another wettable portion until an overflow amount of aqueous liquid is added to the one wettable portion in an amount sufficient to bridge across the mask, and an absorbent material mounted at one side of the surface in position to receive liquid from the slide. The method of use features confining aqueous reagents to the area restricted by the hydrophobic mask, except for when additional amounts are added to force at least some of the liquid to move towards a side edge of the slide, where most of the liquid is absorbed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.