Patent · US Expired

Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays

US5700637A · kind A · utility

911Cited by
10References
8Claims
0Family size

Assignee

Inventor

Key dates

Filing dateApr 19, 1994
Grant dateDec 23, 1997
Priority date
Expiry dateApr 19, 2014

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC40B60/14
  • WIPO fieldOrganic fine chemistry
  • WIPO sectorChemistry

Abstract

This invention provides an apparatus and method for analyzing a polynucleotide sequence; either an unknown sequence or a known sequence. A support, e.g. a glass plate, carries an array of the whole or a chosen part of a complete set of oligonucleotides which are capable of taking part in hybridization reactions. The array may comprise one or more pair of oligonucleotides of chosen lengths. The polynucleotide sequence, or fragments thereof, are labelled and applied to the array under hybridizing conditions. Applications include analyses of known point mutations, genomic fingerprinting, linkage analysis, characterization of mRNAs, mRNA populations, and sequence determination.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.