Identification of differentially expressed genes
US5700644A · kind A · utility
12Cited by
1References
6Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 7, 1995 |
| Grant date | Dec 23, 1997 |
| Priority date | — |
| Expiry date | Jun 7, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6809
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are disclosed to identify differentially expressed genes. In one aspect, one uses subtractive hybridization to enrich for candidate genes, followed by a PCR amplified radiolabeled display of cDNA products of the hybridization. Specially modified primer binding regions are used that can be achieved either through use of trimming plasmids or non-symmetric display plasmids.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.