Vectors and vector systems including genes encoding tumor suppressor proteins and producer cells transformed thereby

US5700657A · kind A · utility

Assignee

• Genzyme Corporation · US

Inventors

• Gary A. Beaudry · Montclair, US
• Arthur Bertelsen · Ridgewood, US
• Michael I. Sherman · Glens Falls, US
• Bert Vogelstein · Baltimore, US

Key dates

• Filing date: Dec 13, 1993
• Grant date: Dec 23, 1997
• Expiry date: Dec 13, 2013

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2799/027
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Vectors and vector systems are embodied that include a gene encoding a tumor suppressor protein. In one embodiment, the vector includes a gene encoding a tumor suppressor protein and an inducible promoter controlling the gene encoding a tumor suppressor protein. The tumor suppressor protein may be p53 protein. In another embodiment, there is provided a vector system which comprises a first vector including a gene encoding a tumor suppressor protein, and a second vector containing a nucleic acid sequence encoding an antisense sequence complementary to all or a portion of the gene encoding a tumor suppressor protein. In yet another embodiment, there is provided a vector system which comprises a first vector including a gene encoding a tumor suppressor protein, and a second vector containing a gene which encodes a protein which binds to, and thereby inhibits, the tumor suppressor protein. Such vectors and vector systems enable cells transformed thereby to continue to proliferate such that adequate amounts of infectious viral particles, generated from viral vectors including a gene encoding a tumor suppressor protein, can be harvested for administration to patients.