Strategy for the production of RNA from immobilized templates
US5700667A · kind A · utility
37Cited by
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46Claims
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Assignee
Inventors
Key dates
| Filing date | May 18, 1995 |
| Grant date | Dec 23, 1997 |
| Priority date | — |
| Expiry date | May 18, 2015 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01F33/45
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The instant invention provides a method for producing RNA enzymatically in a semi-batch or continuous-flow bioreactor using a DNA template immobilized to a solid support through a linkage having a single-stranded overhang extending from a noncoding strand. The coding strand remains dissociable from the immobilized noncoding strand. The immobilized RNA template is reused in at least sixteen rounds of transcription reaction. The coding strand can be replaced with a fresh DNA strand encoding the same or a different RNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.