Patent · US Expired

Nucleoside analogue method

US5702925A · kind A · utility

6Cited by
1References
20Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMay 24, 1996
Grant dateDec 30, 1997
Priority date—
Expiry dateMay 24, 2016

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12P19/30
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A method is provided of making a dideoxynucleoside mono-or tri-phosphate, which is optionally 32-P or 33-P or 35-S radiolabelled which involves reacting the corresponding dideoxynucleoside with an optionally radiolabelled nucleotide phosphate or thiophosphate donor in the presence of a kinase of phosphotransferase enzyme which catalyses the reaction. A method of sequencing nucleic acids by a chain termination of a kinase or phosphotransferase enzyme which catalyses the reaction. A method of sequencing nucleic acids by a chain termination technique involves detecting the products of enzymatic synthesis by means of isotopically labelled chain terminating nucleotide analogues.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.