Mutagenesis methods and compositions
US5702931A · kind A · utility
11Cited by
2References
12Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 28, 1993 |
| Grant date | Dec 30, 1997 |
| Priority date | — |
| Expiry date | Dec 28, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/85
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and reagents for oligonucleotide-directed mutagenesis of a target nucleic acid are provided. In these methods a mutagenic oligonucleotide introduces a desired mutation at one site and, at a second site, introduces or eliminates a restriction site, allowing one to screen for the desired mutation by restriction analysis. Also provided are vectors and kits for performing such mutagenesis methods.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.