Culture vessel for cell cultures on a carrier
US5702945A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 1, 1996 |
| Grant date | Dec 30, 1997 |
| Priority date | — |
| Expiry date | Feb 1, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/40
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The purpose of the present invention is to attain optimal gas exchange, nutrient supply and disposal of metabolic waste products without contamination during high-density cell cultivation of adherent cells. The culture vessel of the present invention provides methods and apparatus for the cultivation of cells on a three-dimensional carrier in a closed cell culture chamber, the supply with oxygen taking place across a gas-permeable gas exchange membrane bordering the cell culture chamber and the supply with nutrients taking place across a dialysis membrane bordering the cell culture chamber, across which nutrients are transported and across which metabolic products are removed, without the danger of contaminating the cells during contact with the tools necessary for manually separating the cells being cultivated from the nutrient medium. The cell culture chamber of the present invention features a vessel that is impermeable to fluids on all sides except for the boundary formed by the dialysis membrane. This makes it possible to move the cell culture intensively within a fluid nutrient supply medium. It is neither necessary to open the cell culture chamber during cultivation to intro…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.