Solid phase synthesis of oligonucleotides with stereospecific substituted phosphonate linkages by pentavalent grignard coupling
US5703223A · kind A · utility
9Cited by
4References
19Claims
0Family size
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Inventors
Key dates
| Filing date | Sep 2, 1994 |
| Grant date | Dec 30, 1997 |
| Priority date | — |
| Expiry date | Sep 2, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H21/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The present invention provides a method for producing an oligonucleotide having stereospecific substituted phosphonate linkages by use of a pentavalent Grignard coupling reaction on a solid phase support. The method can be used for automated synthesis of oligonucleotides having sequential equatorial or axial stereospecific substituted phosphonate linkages.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.