Target-specific, cytotoxic, recombinant pseudomonas exotoxin

US5705163A · kind A · utility

Assignee

• THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE DEPARTMENT OF HEALTH AND HUMAN SERVICES, NATIONAL INSTITUTES OF HEALTH · US

Inventors

• Ira H. Pastan · Potomac, US
• Vijay K. Chaudhary · Rockville, US
• David Fitzgerald · Rockville, US

Key dates

• Filing date: Jun 5, 1995
• Grant date: Jan 6, 1998
• Expiry date: Jun 5, 2015

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/875
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A target-specific, cytotoxic, recombinant Pseudomonas exotoxin is described. Such toxins are made by inserting specific recognition molecules at specific cloning sites in at least domain III near the carboxyl terminus of the PE molecule. Various modifications of the carboxyl terminus of the PE molecule to increase cytotoxicity are set forth. Multifunctional, recombinant, cytotoxic fusion proteins containing at least two different recognition molecules are provided for killing cells expressing receptors to which the recognition molecules bind with specificity. Methods for producing novel recombinant PE molecules with specific properties are described.