Uses for DNA structure-specific recognition protein

US5705334A · kind A · utility

Assignee

• Massachusetts Institute of Technology · US

Inventors

• Stephen J. Lippard · Cambridge, US
• John M. Essigmann · Cambridge, US
• Brian Donahue · Menlo Park, US
• Jeffrey H. Toney · Westfield, US
• Suzanne Bruhn · Cambridge, US
• Pieter M. Pil · New Haven, US
• Steven Brown · Village of La Jolla, US
• Patti J. Kellett · Cincinnati, US

Key dates

• Filing date: Oct 25, 1994
• Grant date: Jan 6, 1998
• Expiry date: Oct 25, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/142
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods disclosed herein capitalize on the ability of DNA Structure Specific Recognition Proteins (SSRPs) to bind to genomic lesions formed by chemotherapeutic agents, particularly cisplatin-type agents. Methods are provided for predicting whether an agent that damages DNA will also be cytotoxic, and for predicting whether particular eukaryotic cells will be susceptible to killing by a genotoxic drug. A screening method is provided for identifying new genotoxic drugs that produce SSRP-recognized lesions in DNA. Methods also are provided for sensitizing particular eukaryotic cells to killing by chemotherapeutic agents, particularly cisplatin-type drugs.