Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
US5705366A · kind A · utility
42Cited by
10References
15Claims
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Key dates
| Filing date | Sep 15, 1994 |
| Grant date | Jan 6, 1998 |
| Priority date | — |
| Expiry date | Sep 15, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6848
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Multiple target nucleic acids are amplified using polymerase chain reaction in the presence of a nonionic, polymeric volume exclusion agent. The amplification efficiency of low copy target nucleic acids is increased in the presence of the volume exclusion agent even though reduced primer levels are used. In this manner, amplification efficiency of a given target nucleic acid can be more readily manipulated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.