DNA purification and isolation using magnetic particles
US5705628A · kind A · utility
389Cited by
5References
29Claims
0Family size
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Key dates
| Filing date | Sep 20, 1994 |
| Grant date | Jan 6, 1998 |
| Priority date | — |
| Expiry date | Sep 20, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of separating polynucleotides, such as DNA, RNA and PNA, from a solution containing polynucleotides by reversibly and non-specifically binding the polynucleotides to a solid surface, such as a magnetic microparticle, having a functional group-coated surface is disclosed. The salt and polyalkylene glycol concentration of the solution is adjusted to levels which result in polynucleotide binding to the magnetic microparticles. The magnetic microparticles with bound polynucleotides are separated from the solution and the polynucleotides are eluted from the magnetic microparticles.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.