Sensors for measuring analyte concentrations and methods of making same

US5707502A · kind A · utility

Assignee

• Chiron Diagnostics Corporation · US

Inventors

• Robert R. McCaffrey · Franklin, US
• Katarina Tkacik · Concord, US
• Brian Holman · Los Angeles, US
• James E. Flaherty · Laguna Hills, US
• Josef Brown · Salem, US
• Peter G. Edelman · Mukilteo, US

Key dates

• Filing date: Jul 12, 1996
• Grant date: Jan 13, 1998
• Expiry date: Jul 12, 2016

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/817
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A sensor for measuring a concentration of an analyte in a solution. The sensor comprises a substrate layer, an electrode layer and an immobilized enzyme layer. The sensor further includes an enzyme/polymer layer and/or a hydrophobic layer. The enzyme/polymer layer includes an enzyme disposed within a polymer matrix. The hydrophobic layer is formed from a material that is more hydrophobic than the immobilized enzyme layer material. In certain embodiments, the sensor includes the enzyme/polymer layer and the hydrophobic layer disposed between the enzyme/polymer layer and the immobilized enzyme layer such that the enzyme/polymer layer is disposed along the hydrophobic layer within an area above the immobilized enzyme layer.