Process for the production and refolding of unfused IGF-II protein in E. coli
US5708148A · kind A · utility
8Cited by
4References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 16, 1994 |
| Grant date | Jan 13, 1998 |
| Priority date | — |
| Expiry date | Nov 16, 2014 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A process for the preparation of a recombinant IGF-II (rIGF-II) without a covalently attached foreign protein moiety and without N-terminal attached methionine or a derivative of methionine or of a salt of said IGF-II, rIGF-II produced by said method, hybrid vectors comprising DNA encoding said rIGF-II, hosts transformed with said vectors, and a process for the isolation of said rIGF-II from the host cell and refolding it into a biologically active form.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.