Tissue formation by injecting a cell-polymeric solution that gels in vivo
US5709854A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 30, 1993 |
| Grant date | Jan 20, 1998 |
| Priority date | — |
| Expiry date | Apr 30, 2013 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61L2400/06
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A cell-polymeric solution is injected into an animal where the polymer crosslinks to form a polymeric hydrogel containing dispersed cells and the cells form new tissue in the animal. The polymer is biodegradable and is a natural polymer such as alginate or a synthetic polymer. The cells are chondrocytes, osteoblasts, muscle cells, fibroblasts or cells acting primarily to synthesize, secret or metabolize materials. Crosslinking of the polymer results from using cations or anions, altering the pH or changing the temperature. A polyion such as polyethyleneimine or polylysine can be added before injection to stabilize the polymeric hydrogel. A kit for tissue formation is provided by combining the cell-polymeric solution with a means for injecting the solution into an animal.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.