Process and vector for expressing alpha-interferon in E. coli

US5710027A · kind A · utility

Assignee

• Boehringer Ingelheim International GmbH · DE

Inventors

• Rudolf Hauptmann · Ebreichsdorf, AT
• Edgar Falkner · Wien, AT
• Gerhard Bodo · Wien, AT
• Tilman Voss · Mödling, AT
• Ingrid Maurer-Fogy · Wien, AT

Key dates

• Filing date: May 26, 1994
• Grant date: Jan 20, 1998
• Expiry date: May 26, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and vectors for expressing interferon-alpha (IFN-.alpha.) proteins in E. coli are provided. Use of a vector comprising an IFN-.alpha. sequence fused to an E. coli heat-stable enterotoxin signal sequence (STII) under the control of the E. coli phosphatase (phoA) promoter affords high levels of correctly-folded and -processed, biologically active IFN-.alpha. polypeptides.