Oligoribonucleotide assays for novel antibiotics

US5712096A · kind A · utility

Assignee

• University of Massachusetts Medical Center · US

Inventors

• Seth Stern · Palo Alto, US
• Prakash C. Purohit · Minneapolis, US

Key dates

• Filing date: Jul 5, 1995
• Grant date: Jan 27, 1998
• Expiry date: Jul 5, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6888
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The oligoribonucleotide analogs of the invention are relatively small, three-dimensional structures derived from larger parental RNA molecules. The analogs include a first nucleic acid structure including one or more nucleotide sequences that are derived from a region of parental RNA, wherein in its native state, the region binds to a ligand, e.g., an aminoglycoside, with a parental RNA ligand binding pattern, and a second nucleic acid structure including one or more nucleotide sequences combined with the first nucleic acid structure to form the analog and provide the analog with a conformation that binds the ligand with a ligand binding pattern that is substantially identical to the parental RNA ligand binding pattern. These analogs can be used to identify novel therapeutic compounds.