Compositions for expression of proteins in host cells using a preprocollagen signal sequence
US5712114A · kind A · utility
31Cited by
6References
39Claims
0Family size
Assignee
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Key dates
| Filing date | Jun 6, 1995 |
| Grant date | Jan 27, 1998 |
| Priority date | — |
| Expiry date | Jun 6, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Hybrid genes comprising a nucleotide sequence encoding a preprocollagen signal sequence operatively linked to a nucleotide sequence encoding a heterologous protein are disclosed. The hybrid genes are useful for secretion of heterologous proteins from host cells. In a preferred embodiment, the signal sequence of the human preprocollagen .alpha.1(I) protein is linked to a heterologous protein of interest to allow for secretion of the protein from Hansenula polymorpha cells.
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