Process for transforming monocotyledonous plants
US5712135A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 7, 1995 |
| Grant date | Jan 27, 1998 |
| Priority date | — |
| Expiry date | Jun 7, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8289
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process for the stable integration of a DNA comprising a gene that is functional in a cell of a cereal plant wherein the DNA is integrated into the nuclear genome of a cereal plant, the process comprising: PA1 (a) providing a compact embryogenic callus of the cereal plant, PA1 (b) wounding the compact embryogenic callus by cutting the callus into pieces or treating the compact embryogenic callus with a cell wall degrading enzyme for a period of time so as not to cause a complete disruption of tissues and transferring the DNA into the nuclear genome of a cell in the compact embryogenic callus by means of electroporation to generate a transformed cell, and PA1 (c) regenerating a transformed cereal plant from the transformed cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.