Method for increasing thermostability in cellulase ennzymes
US5712142A · kind A · utility
10Cited by
5References
13Claims
0Family size
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Key dates
| Filing date | Feb 22, 1996 |
| Grant date | Jan 27, 1998 |
| Priority date | — |
| Expiry date | Feb 22, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02E50/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The gene encoding Acidothermus cellulolyticus E1 endoglucanase is cloned and expressed in Pichia pastoris. A new modified E1 endoglucanase enzyme comprising the catalytic domain of the full size E1 enzyme demonstrates enhanced thermostability and is produced by two methods. The first method of producing the new modified E1 is proteolytic cleavage to remove the cellulose binding domain and linker peptide of the full size E1. The second method of producing the new modified E1 is genetic truncation of the gene encoding the full size E1 so that the catalytic domain is expressed in the expression product.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.