Glucose responsive insulin secreting .beta.-cell lines and method for producing same
US5712159A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 5, 1995 |
| Grant date | Jan 27, 1998 |
| Priority date | — |
| Expiry date | Jun 5, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2500/14
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A cell culture of correctly regulated .beta.-cells having enhanced secretion of insulin is described. A method of selecting such correctly regulated .beta.-cells is also described comprising the following steps: PA0 (a) providing a population of cells comprising .beta.-cells in which increased intracellular concentrations of calcium ions is correlated with the extracellular presence of glucose; PA0 (b) exposing the population to a vital calcium-activated labelling agent; PA0 (c) exposing the population to glucose in a concentration sufficient to result in secretion of the insulin; and PA0 (d) selecting from the population, cells which exhibit a higher level of intracellular free calcium or insulin secretion when exposed to said concentration.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.