Chemically defined cell culture media and system and methods for use, particularly for culturing epithelial cells
US5712163A · kind A · utility
77Cited by
9References
27Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 28, 1995 |
| Grant date | Jan 27, 1998 |
| Priority date | — |
| Expiry date | Mar 28, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/54
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides systems, methods and chemically defined media for the cultivation of cells, particularly epithelial cells. Cells may be cultured with a varied calcium concentration. Furthermore, a calcium concentation in excess of 1.00 mM may be used in the practice of the present invention without loss of a proliferative cell population and maintianing high colony forming efficiencies. Population doubling times range from about 16 to about 33 hours. Cells may serially cultivated to achieve from about 20 to about 50 population doublings.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.