Immunoassay elements having a receptor zone

US5714340A · kind A · utility

Assignee

• Johnson & Johnson Clinical Diagnostics, Inc. · US

Inventors

• Richard C. Sutton · Rochester, US
• Margaret J. Smith-Lewis · Pittsford, US
• Linda A. Mauck · Rochester, US
• Wayne A. Bowman · Walworth, US
• Susan J. Danielson · Rochester, US

Key dates

• Filing date: Jun 16, 1994
• Grant date: Feb 3, 1998
• Expiry date: Jun 16, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/81
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A dry immunoassay analytical element, for assaying a ligand is disclosed. The element comprises, in the following order, (a) a layer containing a labeled ligand, (b) a bead spreading layer, c) a cross-linked hydrophilic polymer layer and d) a support; wherein (i) a fixed concentration of an immobilized receptor for the labeled ligand is located in a zone at the interface of layers (b) and (c); and (ii) the receptors are immobilized by being covalently bonded to polymeric beads that are smaller than the beads in layer (c).