Fumonisin detoxification enzymes
US5716820A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 26, 1997 |
| Grant date | Feb 10, 1998 |
| Priority date | — |
| Expiry date | Feb 26, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8282
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for identifying organisms capable of degrading fumonisin. Fumonisin can be incorporated into culture medium for selection of organisms resistant to fumonisin. Using this method, several organisms have been identified. These organisms can be used to isolate the enzyme and the gene responsible for conferring fumonisin-resistance. The gene can be cloned and inserted into a suitable expression vector so that the protein can be further characterized. Additionally, the DNA encoding for fumonisin-resistance can be used to transform plant cells normally susceptible to Fusarium infection. Plants can be regenerated from the transformed plant cells. In this way, a transgenic plant can be produced with the capability of degrading fumonisin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.