Chimaeric gene coding for a transit peptide and a heterologous peptide

US5717084A · kind A · utility

Assignees

• Plant Genetic Systems N.V. · BE
• Bayer A.G. · DE

Inventors

• Luis Rafael Herrera-Estrella · Evergem, BE
• Guido Van Den Broeck · Evergem, BE
• Marc Van Montagu · Brussels, BE
• Peter Schreier · Cologne, DE
• Jeff Schell · Cologne, DE
• Hans J. Bohnert · Tucson, US
• Anthony R. Cash more · New Rochelle, US
• Michael Paul Timko · Charlottesville, US
• Albert P. Kausch · Stonington, US

Key dates

• Filing date: Jun 6, 1995
• Grant date: Feb 10, 1998
• Expiry date: Jun 6, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8221
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A chimaeric DNA sequence comprising: PA1 a) a nucleic acid sequence coding for a chimaeric protein or polypeptide comprising in sequence: PA2 1) a transit peptide of a cytoplasmatic precursor of a chloroplast protein or polypeptide of a plant species, and, PA2 2) a protein or polypeptide of interest which is different from a mature chloroplast protein or polypeptide contained in a natural cytoplasmatic precursor thereof, and, PA1 b) a promoter upstream of the nucleic acid sequence recognized by polymerases endogenous to a plant, whereby the chimaeric DNA sequence can be expressed in cells of the plant under the control of the promoter and the protein or polypeptide of interest can be translocated in the chloroplasts of the cells of the plant.