In situ hybridization method
US5719023A · kind A · utility
12Cited by
2References
44Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 3, 1994 |
| Grant date | Feb 17, 1998 |
| Priority date | — |
| Expiry date | Jun 3, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of identifying the presence of a known target sequence in nucleic acid contained in a fixed cellular or subcellular biological structure. By adding a stable, reporter-labeled RecA/single-stranded probe complex to the cellular or subcellular structure, the target sequence can be effectively labeled by in situ hybridization, allowing the target sequence to be visualized histologically and microscopically or detected by in situ cytometry or cell sorting flow techniques.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.