Sulfonamide resistance genes and their use
US5719046A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 19, 1995 |
| Grant date | Feb 17, 1998 |
| Priority date | — |
| Expiry date | May 19, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Plant cells are transformed by: PA1 (i) transforming a plant cell whose growth is sensitive to inhibition by a sulfonamide or a salt thereof with a chimaeric gene comprising (a) a plant promoter, (b) a sulfonamide resistance gene having a sequence encoding a transit peptide fused to the 5'-end of the resistance gene and (c) a plant polyadenylation/terminator sequence; PA1 (ii) selecting a transformed plant cell whose growth is resistant to inhibition by a sulfonamide or salt thereof; PA1 (iii) optionally, regenerating from the transformed plant cell a genetically transformed plant which exhibit the said resistance; PA1 (iv) optionally, obtaining seed from the regenerated plant; and PA1 (v) optionally, propagating plants from the seed. The growth of weeds can be controlled at a locus where a transgenic plant obtained as above is being cultivated by applying to the locus an effective amount of a herbicide, such as asulam, which acts by inhibiting dihydropteroate synthase. The sulfonamide resistance gene can be also used as a selectable marker. The sequence of a sulfonamide resistance gene is presented.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.