Expression system for producing apolipoprotein AI-M

US5721114A · kind A · utility

Assignee

• Pharmacia & Upjohn Company LLC · US

Inventors

• Lars Abrahamsén · Stockholm, SE
• Erik Holmgren · Lidingö, SE
• Christina Kalderen · Stockholm, SE
• Mats Lake · Lidingö, SE
• Åsa Mikaelsson · Stockholm, SE
• Torsten Sejlitz · Solna, SE

Key dates

• Filing date: Aug 25, 1995
• Grant date: Feb 24, 1998
• Expiry date: Aug 25, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to an expression system giving high extracellular production of apolipoprotein AI-M (Milano) using E. coli and comprises a plasmid carrying an origin of replication, an inducible promoter sequence, a DNA sequence coding for a signal peptide, a DNA sequence coding for apolipoprotein AI-M, and a transcription terminator. The invention also relates to a method of producing apolipoprotein AI-M using the expression system.