Method of attaching a fluorescent label to an oligonucleotide
US5721355A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 25, 1996 |
| Grant date | Feb 24, 1998 |
| Priority date | — |
| Expiry date | Jun 25, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/55
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Compounds useful to attach a fluorescein label to an oligonucleotide are disclosed. To create these compounds, oxygen groups on the fluorescein moiety are protected (e.g. with acyl groups). One then links a phosphoramidite to the fluorescein moiety via an amide or thiourea linkage to an active site on the fluorescein ring. The resulting compound can then be directly linked to an oligonucleotide as it is being formed in an automated synthesizer and then deprotected using the same deprotection conditions as are used to deprotect the oligonucleotides. Such compounds are, inter alia, useful to create labelled primers for DNA sequencing.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.