Method for the analytical separation of viruses

US5723031A · kind A · utility

Assignee

• BAYER HEALTHCARE LLC · DE

Inventors

• Hansjorg Durr · Burscheid, DE
• Hans-Robert Hehnen · Siegburg, DE
• Lothar Helbig · Leverkusen, DE
• Roberto Correa · Leverkusen, DE
• Ulf Bruggemeier · Leichlingen, DE

Key dates

• Filing date: Oct 24, 1995
• Grant date: Mar 3, 1998
• Expiry date: Oct 24, 2015

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N27/447
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The method permits the non-destructive analytical detection and/or the quantification of viruses or viral particles (analytes) in a liquid sample matrix containing organic or inorganic minor constituents, in particular protein moieties and/or nucleotides and/or other viruses. To this end the viruses or the viral particles are separated from the protein moieties and/or nucleotides in the sample matrix using capillary electrophoresis and at the same time the electropherogram associated therewith is recorded. The fractions assigned to the analytes can then be identified as virus peaks in the electropherogram by spectroscopic interpretation with the aid of characteristic maxima. For better identification, a reference sample of a known virus or of a known viral particle can be added to the sample matrix.