Method of fluorescent detection of nucleic acids and cytoskeleton elements using bis-dicationic aryl furans, and kits useful therefor

US5723288A · kind A · utility

Assignees

• The University of North Carolina at Chapel Hill · US
• Georgia State University Research Foundation · US

Inventors

• Christine C. Dykstra · Chapel Hill, US
• Richard R. Tidwell · Pittsboro, US
• David W. Boykin · Atlanta, US
• W. David Wilson · Atlanta, US
• Jaroslaw Spychala · Poznań, PL
• Bijan P. Das · Atlanta, US
• Arvind Kumar · Atlanta, US

Key dates

• Filing date: May 6, 1994
• Grant date: Mar 3, 1998
• Expiry date: May 6, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/81
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Disclosed is a method of fluorescent detection of a nucleic acid. The method comprises contacting to the nucleic acid a bis-dicationic aryl furan compound, such as 2,5-bis4-(4,5,6,7-tetrahydro-1H-1,3-diazepin-2-yl) phenyl! furan; 2,5-bis{4-(N-isopropyl) amidino! phenyl}furan; and physiologically acceptable salts thereof, and exposing the nucleic acid to light at a frequency to induce fluorescence of the compound. A method for fluorescent detection of cytoskeleton elements, and novel bis-dicationic aryl furan compounds are also disclosed.