Methods for detection and discrimination of multiple analytes using fluorescent technology
US5723294A · kind A · utility
32Cited by
4References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 5, 1996 |
| Grant date | Mar 3, 1998 |
| Priority date | — |
| Expiry date | Mar 5, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6846
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides methods and apparatus for detecting and discriminating multiple analytes within a test sample which are rapid, accurate, and convenient enough for routine use in a clinical laboratory. In particular, the present invention provides methods and apparatus for permitting multiple PCR-amplified target nucleic acid sequence hybrids within a single sample, labeled with different fluorescent dyes, to be spectrally distinguished using the read-out data directly from a fluorescence reader instrument.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.